Standard Test Methods for
Autoclavability of Membrane Filters1

This standard is issued under the fixed designation D 4199; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.

1. Scope

1.1 These test methods deal with tests to determine the
autoclavability of membrane filters.

1.2 These test methods are applicable to all membrane
filters. Either Test Method A, Test Method B, or both, may be
utilized to assess the autoclavability of membrane filters.
Section
Test Method A—Dimensional Stability 8.1
Test Method B—Wetting Characteristics 8.2

1.3 This standard does not purport to address all of the
safety concerns associated with its use, if any. It is the
responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2. Referenced Documents

2.1 ASTM Standards: 2
D 1129 Terminology Relating to Water
D 1193 Specification for Reagent Water

3. Terminology

3.1 Definitions—For definitions of terms used in these test
methods, refer to Terminology D 1129.

4. Summary of Test Methods

4.1 Test Method A— Autoclavability is evaluated by measuring the diameter of the membrane filter, across three axes,
prior to autoclaving and after autoclaving, to determine dimensional stability.

4.2 Test Method B— Wettability is determined by placing
samples of autoclaved and non autoclaved membrane filters in
water and measuring the time required for the filters to become
completely wet.

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