Standard Classification for
Sampling Phytoplankton in Surface Waters1

This standard is issued under the fixed designation D 4149; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.

1. Scope

1.1 This classification covers both qualitative and quantitative techniques that are used commonly for the collection of
phytoplankton. The particular techniques that are used during
an investigation are dependent upon the study objectives. Of
additional importance in the selection of a technique is the
uneven distribution of organisms both temporally and spacially. This classification describes qualitative and quantitative
ways of collecting phytoplankton from inland surface waters.
Specifically, qualitative samplers include conical tow nets and
pumps; quantitative samplers include the Clarke-Bumpus
plankton sampler, Juday plankton trap, water sampling bottles,
and depth-integrating samplers.

2. Referenced Documents

2.1 ASTM Standards:
D 1129 Terminology Relating to Water2

3. Terminology

3.1 Definitions:

3.1.1 For definitions of terms used in this method refer to
Terminology D 1129.

3.2 Definitions of Terms Specific to This Standard:

3.2.1 phytoplankton—is the community of suspended or
floating, mostly microscopic plants that drift passively with
water currents. Frequently, phytoplankton are differentiated on
the basis of size. The generally accepted size ranges, as
commonly used are (1):3
Macroplankton >500 µm
Microplankton (net plankton) 10 to 500 µm
Nannoplankton 10 to 50 µm

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