Standard Practice for
Sampling Fish with Rotenone1

This standard is issued under the fixed designation D 4131; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.

1. Scope

1.1 This practice covers determination of the quantitative
and qualitative species composition of fish in a specified area.
The successful use of this technique is dependent on: (1)
preventing fish from escaping the sample area and (2) retrieving all affected fish, which may take up to three days.

1.2 Advantages:

1.2.1 Easily detoxified.

1.2.2 All native freshwater fish are susceptible, but it has
low toxicity to mammals and birds.

1.2.3 At low concentrations fish toxicity depends on species, age, and size.

1.2.4 The suffocating action is reversible.

1.3 Limitations:

1.3.1 It is less effective in cold (below 20°C) and highly
alkaline water.

1.3.2 Smaller fish and those without air bladders usually do
not float.

1.3.3 Completely random selection of sample areas is not
possible.

1.3.4 Overkill beyond sample area can sometimes occur.

1.3.5 Food web organisms may be eliminated.

1.4 Applications—this practice is useful in both short- and
long-term studies for management and impact assessment
purposes. It is adaptable to both lotic and lentic situations in
littoral and limnetic areas.

1.5 The values stated in inch-pound units are to be regarded
as the standard. The values given in parentheses are for
information only.

1.6 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific
hazards, see Section 6.

2. Referenced Document

2.1 ASTM Standards:

Click below to download Astm D 4131 – 84 (Reapproved 1999)e1 pdf free

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Click here to download Astm D 4130 – 03 Pdf Free Download