Classification for
Fish Sampling1

This standard is issued under the fixed designation D 4211; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.

1. Scope

1.1 This classification covers rotenone and antimycin which
are used to collect or eradicate fish; numerous chemicals have
been used but presently only rotenone and antimycin are EPA
approved for this use.

2. Referenced Document

2.1 ASTM Standards: 2
D 4131 Practice for Sampling Fish with Rotenone

3. Significance and Use

3.1 The significance of using chemical fish toxicants is that
more complete population analyses or total eradication, or
both, can be accomplished. Target species can be selectively
eradicated by varying concentrations. This provides a very
effective tool in fisheries investigations and management
programs. Water conditions (that is, pH, temperature, alkalinity, and so forth) and morphology can be limiting factors.

3.2 Rotenone—Rotenone used as a fish toxicant is highly
versatile and can be used effectively to collect fish samples; to
eradicate fish; and to selectively remove certain fish species.

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