Standard Practice for
Sampling Phytoplankton With Depth-Integrating Samplers1

This standard is issued under the fixed designation D 4135; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.

1. Scope

1.1 This practice covers the procedures for obtaining quantitative samples of a phytoplankton community by use of
depth-integrating sampler. The method is suitable for use in
flowing waters.

1.2 This standard does not purport to address all of the
safety problems, if any, associated with its use. It is the
responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Fig. 3

2. Referenced Documents

2.1 ASTM Standards: 2
D 4137 Practice for Preserving Phytoplankton Samples

3. Summary of Practice

3.1 A depth-integrated sample is collected by means of a
sampler that integrates stream discharge as a function of depth.
The velocity of flow in a stream, as well the density of
phytoplankton, varies both vertically and horizontally. Depth
integration is used to collect a sample that is weighted
according to the velocity at each increment of depth.3 The
phytoplankton may be preserved as dictated by the objectives
of the study.

4. Significance and Use

4.1 The advantages of the depth-integrating samplers are as
follows:

4.1.1 The samplers provide the only means by which a
representative sample of phytoplankton can be obtained from a
stream.

4.1.2 The variety of sampler designs result in the availability of a sampler that can be used in virtually all velocities of
flow

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