Standard Practice for
Sampling Phytoplankton with Pumps1

This standard is issued under the fixed designation D 4133; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.

1. Scope

1.1 This practice covers the procedures for obtaining qualitative samples of a phytoplankton community by use of
pumping systems.

1.2 This standard does not purport to address all of the
safety problems, if any, associated with its use. It is the
responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2. Referenced Document

2.1 ASTM Standards: 2
D 4137 Practice for Preserving Phytoplankton Samples

3. Summary of Practice

3.1 Water is pumped from a discrete depth and passed
through a net. The captured phytoplankton are removed from
the net and preserved as dictated by the objectives of the study.

4. Significance and Use

4.1 The advantages of a pumping phytoplankton sampler
are as follows:

4.1.1 Sample size is more accurately controlled than with
the use of a conical tow net.

4.1.2 Discrete samples from any depth are easily obtained.

4.1.3 Multiple or replicate samples are easily obtained.

4.1.4 They are adaptable to shallow ecosystems.

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